Spectrophotometric determination of cysteine hydrochloride by phosphomolybdic blue

Xinrong Wen; Changqing Tu

doi:10.1117/12.2640357

29 April 2022 Spectrophotometric determination of cysteine hydrochloride by phosphomolybdic blue

Xinrong Wen, Changqing Tu

Proceedings Volume 12255, 2022 International Conference on Optoelectronic Information and Functional Materials (OIFM 2022); 1225528 (2022) https://doi.org/10.1117/12.2640357
Event: 2022 International Conference on Optoelectronic Information and Functional Materials (OIFM 2022), 2022, Chongqing, China

Abstract

In H₂SO₄ medium, phosphorus molybdenum heteropoly acid formed from H₂PO₄ - reacts with Mo₇O₂₄^6- can be reduced by hydrosulfuryl(-SH) in cysteine hydrochloride to from phosphomolybdic blue which its maximum absorption wavelength is 717.2 nm. Beer's law is obeyed between the mass concentration of cysteine hydrochloride and the absorbance of phosphomolybdic blue, and the content of cysteine hydrochloride can be determined based on the measuring of the absorbance of phosphomolybdic blue. A novel method for the spectrophotometric determination of cysteine hydrochloride by phosphomolybdic blue has been established. A good linear relationship is obtained between absorbance and the concentration of cysteine hydrochloride in the range of 0.008160～0.1469 mg/mL, the equation of the linear regression is A=0.0305+9.9918C(mg/mL), and the linear correlation coefficient is 0.9995. This proposed method had been successfully applied to determinate cysteine hydrochloride, and the result agreed well with those by pharmacopoeial method.

Citation Download Citation

Xinrong Wen and Changqing Tu "Spectrophotometric determination of cysteine hydrochloride by phosphomolybdic blue", Proc. SPIE 12255, 2022 International Conference on Optoelectronic Information and Functional Materials (OIFM 2022), 1225528 (29 April 2022); https://doi.org/10.1117/12.2640357

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