Research on three-dimensional topography measurement method of tumor cells based on lensless imaging

Lulu He; Xiaochen Meng; Jiaqi Cui

doi:10.1117/12.2643256

19 December 2022 Research on three-dimensional topography measurement method of tumor cells based on lensless imaging

Lulu He, Xiaochen Meng, Jiaqi Cui

Proceedings Volume 12318, Holography, Diffractive Optics, and Applications XII; 1231826 (2022) https://doi.org/10.1117/12.2643256
Event: SPIE/COS Photonics Asia, 2022, Online Only

Conference Poster

Abstract

In this paper, the method using lensless microscopic imaging techniques, to reconstruct the three-dimensional morphology of tumor cells is given. We demonstrate lensless microscopic platform which only use a CMOS image sensor and a controllable color LED array. Based on the principle of lensless computational imaging, we use the phase recovery algorithm under multi-wavelength and the diffraction tomography algorithm under multi-angle illumination, to reconstruct the three-dimensional morphology of tumor cells. Experiments have verified that this method can achieve large field of view imaging without labeling and reconstruct the three-dimensional morphology of tumor cells, which provides reliable morphological parameters for clinical research and is of great significance to the development of portable medical care.

Citation Download Citation

Lulu He, Xiaochen Meng, and Jiaqi Cui "Research on three-dimensional topography measurement method of tumor cells based on lensless imaging", Proc. SPIE 12318, Holography, Diffractive Optics, and Applications XII, 1231826 (19 December 2022); https://doi.org/10.1117/12.2643256

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