Use of freshly amputated human limbs for pre-clinical evaluation of molecular-targeted fluorescent probes

Logan M. Bateman; Kendra A. Hebert; Samuel S. Streeter; Jenna A. Nunziata; Connor W. Barth; Lei G. Wang; Summer L. Gibbs; Eric R. Henderson

doi:10.1117/12.2650356

14 March 2023 Use of freshly amputated human limbs for pre-clinical evaluation of molecular-targeted fluorescent probes

Logan M. Bateman, Kendra A. Hebert, Samuel S. Streeter, Jenna A. Nunziata, Connor W. Barth, Lei G. Wang, Summer L. Gibbs, Eric R. Henderson

Author Affiliations +

Proceedings Volume 12361, Molecular-Guided Surgery: Molecules, Devices, and Applications IX; 1236109 (2023) https://doi.org/10.1117/12.2650356
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

We have co-developed a first-in-kind model of fluorophore testing in freshly amputated human limbs. Ex vivo human tissue provides a unique opportunity for the testing of pre-clinical fluorescent agents, collection of imaging data, and histopathologic examination in human tissue prior to performing in vivo experiments. Existing pre-clinical fluorescent agent studies rely primarily on animal models, which do not directly predict fluorophore performance in humans and can result in wasted resources and time if an agent proves ineffective in early human trials. Because fluorophores have no desired therapeutic effect, their clinical utility is based solely on their safety and ability to highlight tissues of interest. Advancing to human trials even via the FDA’s phase 0/microdose pathway still requires substantial resources, single-species pharmacokinetic testing, and toxicity testing. In a recently concluded study using amputated human lower limbs, we were able to test successfully a nerve-specific fluorophore in pre-clinical development. This study used systemic administration via vascular cannulization and a cardiac perfusion pump. We envision that this model may assist with early lead agent testing selection for fluorophores with various targets and mechanisms.

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Logan M. Bateman, Kendra A. Hebert, Samuel S. Streeter, Jenna A. Nunziata, Connor W. Barth, Lei G. Wang, Summer L. Gibbs, and Eric R. Henderson "Use of freshly amputated human limbs for pre-clinical evaluation of molecular-targeted fluorescent probes", Proc. SPIE 12361, Molecular-Guided Surgery: Molecules, Devices, and Applications IX, 1236109 (14 March 2023); https://doi.org/10.1117/12.2650356

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