Simultaneous 2-photon and 3-photon multimodal signal acquisition for multiphoton microscopy

Wentao Wu; Christoph Brandt; Shuo Tang

doi:10.1117/12.2649196

25 April 2023 Simultaneous 2-photon and 3-photon multimodal signal acquisition for multiphoton microscopy

Wentao Wu, Christoph Brandt, Shuo Tang

Proceedings Volume 12384, Multiphoton Microscopy in the Biomedical Sciences XXIII; 123840H (2023) https://doi.org/10.1117/12.2649196
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

We report on a simultaneous 2-photon and 3-photon signal acquisition method for label-free multiphoton microscopy. With dual excitation wavelengths of 1580 nm and 790 nm and a set of filter designs, multiple contrasts including second harmonic generation, third harmonic generation, and two-photon-excitation-fluorescence images are detected and separated. The spectrum of the nonlinear signals is measured and verified by a spectrometer. Depth-resolved multimodal images are demonstrated on a silicon photonic chip and leaf samples. The simultaneous 2- and 3-photon microscopy is shown to have great potential for label-free in-vivo imaging.

Conference Presentation

Citation Download Citation

Wentao Wu, Christoph Brandt, and Shuo Tang "Simultaneous 2-photon and 3-photon multimodal signal acquisition for multiphoton microscopy", Proc. SPIE 12384, Multiphoton Microscopy in the Biomedical Sciences XXIII, 123840H (25 April 2023); https://doi.org/10.1117/12.2649196

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