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16 March 2023 Application of spectrophotometry to detect the urea in milk sample
Md Tauseef Iqbal Ansari, Sanjeev Kumar Raghuwanshi, Purnendu Shekhar Pandey, Santosh Kumar
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Proceedings Volume 12396, Plasmonics in Biology and Medicine XX; 123960A (2023) https://doi.org/10.1117/12.2647995
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
Food adulteration is a global concern, and developing countries are under serious threat owing to a lack of supervision and laws. Specially, milk adulterants can cause severe health risks, resulting in fatal diseases. Conventional and qualitative detection techniques are limited due to the more sophisticated way of milk adulteration and involve complexity in the processes. This paper used ultraviolet-visible-near infrared (UV-Vis-NIR) spectrophotometric measurements technique to detect the urea concentration in the milk sample. Urea concentration was taken initially at the steps of 5% and then with 10% to mix with pure milk to measure the adulteration. Results showed that the absorbance spectrum increased proportionally in the Vis and NIR regions when we increased or added the amount of urea to milk. The proposed spectrophotometry method will be a successful basis for the screening of optical wavelength to help the researcher to find out the surface plasmon resonance (SPR) phenomenon where the light and matter interaction is maximum.
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Md Tauseef Iqbal Ansari, Sanjeev Kumar Raghuwanshi, Purnendu Shekhar Pandey, and Santosh Kumar "Application of spectrophotometry to detect the urea in milk sample", Proc. SPIE 12396, Plasmonics in Biology and Medicine XX, 123960A (16 March 2023); https://doi.org/10.1117/12.2647995
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KEYWORDS
Urea
Absorbance
Spectrophotometry
Urea concentration
Light absorption
Light sources
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