Presentation
17 March 2023 Nanoplasmonic isothermal PCR assay with CRISPR/Cas for real-time SARS-CoV-2 detection
Author Affiliations +
Proceedings Volume 12434, MOEMS and Miniaturized Systems XXII; 124340D (2023) https://doi.org/10.1117/12.2650879
Event: SPIE OPTO, 2023, San Francisco, California, United States
Abstract
This paper reports a nanoplasmonic isothermal PCR assay with CRISPR/Cas for real-time SARS-CoV-2 detection. The study utilizes a microchip and a miniaturized hand-held type photothermal PCR system, which comprises a nanoplasmonic photothermal heater and microlens array camera to maintain the temperature and detect fluorescence signal from the chip. CRISPR-based fluorescence signal detection, which proceeds simultaneously with nucleic acid amplification, indicates higher sensitivity and rapid detection. The real-time nanoplasmonic isothermal PCR assay opens a new opportunity for POCT-based CRISPR assay.
Conference Presentation
© (2023) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Eun-Sil Yu, Byoung-Hoon Kang, Hamin Na, and Ki-Hun Jeong "Nanoplasmonic isothermal PCR assay with CRISPR/Cas for real-time SARS-CoV-2 detection", Proc. SPIE 12434, MOEMS and Miniaturized Systems XXII, 124340D (17 March 2023); https://doi.org/10.1117/12.2650879
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KEYWORDS
Nanoplasmonics

Luminescence

Microlens array

Signal detection

Cameras

Eye

Gold

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