Realization of bio-Raman black hair analysis by automatic signal separation of melanin from other hair components

Jianhai He; Kazuki Kobayashi; Mana Nemoto; Mohamed Mubark Abdel Galeil; Kazuyuki Suzuta; Len Ito; Shin-ichi Morita

doi:10.1117/12.3007512

20 September 2023 Realization of bio-Raman black hair analysis by automatic signal separation of melanin from other hair components

Jianhai He, Kazuki Kobayashi, Mana Nemoto, Mohamed Mubark Abdel Galeil, Kazuyuki Suzuta, Len Ito, Shin-ichi Morita

Author Affiliations +

Proceedings Volume 12608, Biomedical Imaging and Sensing Conference; 126080G (2023) https://doi.org/10.1117/12.3007512
Event: Optics and Photonics International Congress, 2023, Yokohama, Japan

Abstract

Raman research on black colored hair has been limitedly reported, because (i) the laser incident frequently burns and destroys the hair; (ii) Raman bands of melanin are much larger and broader, overlapped with the other hair originated Raman signals, which stopped Raman hair analysis. Here, we realized a versatile bio-Raman hair analysis including black colored hair system, (i) by fabricating a semi ultrathin cross section with the thickness of 500 nm of black hair using an ultramicrotome; (ii) by separating the signal of melanin from that of other hair components using the automatic background removal method that recently we developed.

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Jianhai He, Kazuki Kobayashi, Mana Nemoto, Mohamed Mubark Abdel Galeil, Kazuyuki Suzuta, Len Ito, and Shin-ichi Morita "Realization of bio-Raman black hair analysis by automatic signal separation of melanin from other hair components", Proc. SPIE 12608, Biomedical Imaging and Sensing Conference, 126080G (20 September 2023); https://doi.org/10.1117/12.3007512

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