Autofluorescence imaging enhanced by deep-ultraviolet surface plasmon resonance for label-free bio-sensing

K. Hosomi; C. N. H. Che Lah; H. Morisawa; K. Kobayashi; Y. Tanaka; A. Ono; W. Inami; Y. Kawata

doi:10.1117/12.3007931

20 September 2023 Autofluorescence imaging enhanced by deep-ultraviolet surface plasmon resonance for label-free bio-sensing

K. Hosomi, C. N. H. Che Lah, H. Morisawa, K. Kobayashi, Y. Tanaka, A. Ono, W. Inami, Y. Kawata

Author Affiliations +

Proceedings Volume 12608, Biomedical Imaging and Sensing Conference; 1260817 (2023) https://doi.org/10.1117/12.3007931
Event: Optics and Photonics International Congress, 2023, Yokohama, Japan

Abstract

Fluorescence from cells excited by deep-ultraviolet (DUV) light is known as autofluorescence and intrinsic properties of cells. Therefore, the imaging of the distribution of autofluorescence of biological specimen enables us to promote tissue histology and cell biology. This fluorescence is, however, very weak, which prevents the development of autofluorescence imaging. The present work utilizes the surface plasmon resonance (SPR) on aluminum surfaces to enhance the fluorescence signals for highly sensitive imaging of living cells without staining. Aluminum is promising material for the excitation of SPR in the DUV region (DUV-SPR) due to their negative permittivity. DUV-SPR was excited with Kretschmann configuration using a sapphire prism. The DUV-SPR excitation of Al thin film through a sapphire prism was investigated theoretically and experimentally. For living biological specimen, i.e. water-immersed specimens, a high refractive index of the sapphire is suitable for the observation. The autofluorescence intensity through DUV-SPR is increased ~3-fold. Results from this work provide an effective technique for highly sensitive label-free observation of living biological specimens.

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K. Hosomi, C. N. H. Che Lah, H. Morisawa, K. Kobayashi, Y. Tanaka, A. Ono, W. Inami, and Y. Kawata "Autofluorescence imaging enhanced by deep-ultraviolet surface plasmon resonance for label-free bio-sensing", Proc. SPIE 12608, Biomedical Imaging and Sensing Conference, 1260817 (20 September 2023); https://doi.org/10.1117/12.3007931

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