Label-free intra-tissue activity imaging of alveolar organoid with three-dimensional dynamic optical coherence tomography

Rion Morishita; Pradipta Mukherjee; Ibrahim Abd El-Sadek; Toshio Suzuki; Antonia Lichtenegger; YiHeng Lim; Yiqiang Zhu; Shuichi Makita; Yuki Yamamoto; Tetsuharu Nagamoto; Yoshiaki Yasuno

doi:10.1117/12.2670861

11 August 2023 Label-free intra-tissue activity imaging of alveolar organoid with three-dimensional dynamic optical coherence tomography

Rion Morishita, Pradipta Mukherjee, Ibrahim Abd El-Sadek, Toshio Suzuki, Antonia Lichtenegger, YiHeng Lim, Yiqiang Zhu, Shuichi Makita, Yuki Yamamoto, Tetsuharu Nagamoto, Yoshiaki Yasuno

Author Affiliations +

Proceedings Volume 12632, Optical Coherence Imaging Techniques and Imaging in Scattering Media V; 126321C (2023) https://doi.org/10.1117/12.2670861
Event: European Conferences on Biomedical Optics, 2023, Munich, Germany

Abstract

We demonstrate the label-free intra-tissue activity imaging of human induced pluripotent stem cell (hiPSC)-derived alveolar organoid including normal and fibrosis model with three-dimensional Dynamic Optical Coherence Tomography (D-OCT). The volumetric D-OCT data were obtained by a 840-nm spectral domain OCT. And the D-OCT images were obtained by “logarithmic-intensity-variance (LIV)” method, which is sensitive to the magnitude of tissue dynamics. Cystic structures with high-LIV border and mesh-like structures with low LIV were observed in all the organoids. They correspond to alveoli and fibroblast. The LIV images also visualized the abnormal repairing process of the alveolar epithelium.

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Rion Morishita, Pradipta Mukherjee, Ibrahim Abd El-Sadek, Toshio Suzuki, Antonia Lichtenegger, YiHeng Lim, Yiqiang Zhu, Shuichi Makita, Yuki Yamamoto, Tetsuharu Nagamoto, and Yoshiaki Yasuno "Label-free intra-tissue activity imaging of alveolar organoid with three-dimensional dynamic optical coherence tomography", Proc. SPIE 12632, Optical Coherence Imaging Techniques and Imaging in Scattering Media V, 126321C (11 August 2023); https://doi.org/10.1117/12.2670861

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