The application of loop-mediated isothermal amplification (LAMP) in the rapid detection of banana allergen

Shangzhi Hu

doi:10.1117/12.2692199

7 September 2023 The application of loop-mediated isothermal amplification (LAMP) in the rapid detection of banana allergen

Shangzhi Hu

Author Affiliations +

Proceedings Volume 12789, International Conference on Modern Medicine and Global Health (ICMMGH 2023); 127890K (2023) https://doi.org/10.1117/12.2692199
Event: International Conference on Modern Medicine and Global Health (ICMMGH 2023), 2023, Oxford, United Kingdom

Abstract

Bananas (Musa acuminata) have been recognized as a common cause of food allergies worldwide. To avoid allergic reactions in patients with allergies, it is important to avoid eating bananas. Therefore, sensitive and specific banana detection methods are needed to verify bananas in food. Loop-mediated isothermal amplification (LAMP) is a fast and simple DNA-based detection method, which is mainly suitable for field application or on-site analysis and screening of allergens in food production. This work describes the systematic development and selection of LAMP primers based on multiple copy genes of banana. The chemical method used allows the detection of amplified DNA using either direct observation of precipitated products or gel electrophoresis. LAMP based on AJ277278 gene is highly specific for bananas, allowing detection sensitivity of approximately sensitivity of 3.9 × 10⁻³ ng/μL DNA extracting solution. Different banana products can be detected at the same sensitivity level. LAMP enables simple, highly specific and sensitive detection of bananas without the need for expensive analytical equipment.

(2023) Published by SPIE. Downloading of the abstract is permitted for personal use only.

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Shangzhi Hu "The application of loop-mediated isothermal amplification (LAMP) in the rapid detection of banana allergen", Proc. SPIE 12789, International Conference on Modern Medicine and Global Health (ICMMGH 2023), 127890K (7 September 2023); https://doi.org/10.1117/12.2692199

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