Study on the inactivation effect of ultraviolet light from multi-irradiance UV-LEDs on bacteria and the underlying damage

Mengmeng Li

doi:10.1117/12.2688485

16 October 2023 Study on the inactivation effect of ultraviolet light from multi-irradiance UV-LEDs on bacteria and the underlying damage

Mengmeng Li

Author Affiliations +

Proceedings Volume 12792, Eighteenth National Conference on Laser Technology and Optoelectronics; 127920F (2023) https://doi.org/10.1117/12.2688485
Event: Eighteenth National Conference on Laser Technology and Optoelectronics, 2023, Shanghai, China

Abstract

Ultraviolet (UV)-mediated inactivation has been widely used in various fields. The doses needed for inactivation of various pathogenic bacteria were compared, and the bacterial survival state after sterilization was determined. The effective sterilization methods and effect of UV irradiation on DNA structure were explored. In the experiment, a UV-C lamp device with multi irradiance and regular irradiation was designed and assembled for irradiation of bacteria. A variety of bacteria were used under unified culture environment and irradiation conditions, and the sterilization rate was calculated through the proliferation of bacteria after UV irradiation. The irradiation dose and dose curve of each strain to achieve the bactericidal rate were determined. The penetration of UV radiation into bacteria was evaluated through the killing effect of UV radiation on colonies. The differences among bacteria and UV energy amounts is discussed herein, and the influence of irradiance on the inactivation effect was demonstrated. Irradiance and irradiation dose play a decisive role in the efficacy of UV sterilization. The UV dose was positively correlated with the bactericidal rate; the bactericidal rate increased with increasing dose. High-dose UV irradiation could kill multilayer bacteria. Detection of bacterial damage showed that the UV energy in the experiment did not break the DNA fragments to yield small-molecular bands. The ultramicrostructure diagram showed that UV irradiation caused condensation of the linear DNA, and the abnormal DNA structure could not replicate or be inherited. The application of UV sterilization requires a suitable energy range.

(2023) Published by SPIE. Downloading of the abstract is permitted for personal use only.

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Mengmeng Li "Study on the inactivation effect of ultraviolet light from multi-irradiance UV-LEDs on bacteria and the underlying damage", Proc. SPIE 12792, Eighteenth National Conference on Laser Technology and Optoelectronics, 127920F (16 October 2023); https://doi.org/10.1117/12.2688485

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KEYWORDS

Ultraviolet radiation

Bacteria

Radiation effects

Lamps

Liquids

Pathogens

Safety

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