Single microvessel occlusion technology (PLP) produces cortical microvasculature redistribution and neuronal network functional adaptation

Wang Xi; Liang Zhu; Hequn Zhang; Mengqi Wang; Anna Roe

doi:10.1117/12.3012512

13 March 2024 Single microvessel occlusion technology (PLP) produces cortical microvasculature redistribution and neuronal network functional adaptation

Wang Xi, Liang Zhu, Hequn Zhang, Mengqi Wang, Anna Roe

Author Affiliations +

Proceedings Volume 12829, Optogenetics and Optical Manipulation 2024; 128290J (2024) https://doi.org/10.1117/12.3012512
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Recent studies have highlighted the importance of understanding the architecture and function of microvasculature, and dysfunctions of these microvessels may underlie neurodegenerative disease. Here, we utilized a highly precision ultrafast laser-induced photothrombosis (PLP) method to occlude single capillaries and then quantitatively studied effects on vasodynamics and surrounding neurons. Analysis of the microvascular architecture and hemodynamics after single-capillary occlusion reveals distinct changes upstream vs downstream branches, which shows rapid regional flow redistribution and local downstream BBB leakage. Focal ischemia via capillary occlusions surrounding labeled target neuron induced dramatic and rapid lamina-specific changes in neuronal dendritic architecture. The adaptive changes in neuronal function networks were correlated to the degree of ischemia core. Further, we find that micro-occlusion at two different depths within the same vascular arbor results in distinct effects on flow profiles in layerin layer 2/3 vs layer 4. The current results raised the possibility that relatively greater impacts on microvascular function contribute to neuronal network function.

Conference Presentation

Citation Download Citation

Wang Xi, Liang Zhu, Hequn Zhang, Mengqi Wang, and Anna Roe "Single microvessel occlusion technology (PLP) produces cortical microvasculature redistribution and neuronal network functional adaptation", Proc. SPIE 12829, Optogenetics and Optical Manipulation 2024, 128290J (13 March 2024); https://doi.org/10.1117/12.3012512

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KEYWORDS

Capillaries

Ischemia

Vascular diseases

Pathophysiology

Reproducibility

Therapeutics

Two photon imaging

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