A study on mitochondrial dynamics and membrane potential in mammalian cells using home-built simultaneous multiple-level magnification selective plane illumination microscopy (sMx-SPIM)

Aiswarya K.S.; Nikhil Dev Narendradev; Piyao Narumbam; Parul Jain; Srinivasa Murty Srinivasula; Mayanglambam Suheshkumar Singh

doi:10.1117/12.3003659

12 March 2024 A study on mitochondrial dynamics and membrane potential in mammalian cells using home-built simultaneous multiple-level magnification selective plane illumination microscopy (sMx-SPIM)

Aiswarya K.S., Nikhil Dev Narendradev, Piyao Narumbam, Parul Jain, Srinivasa Murty Srinivasula, Mayanglambam Suheshkumar Singh

Author Affiliations +

Proceedings Volume 12846, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXII; 128460E (2024) https://doi.org/10.1117/12.3003659
Event: SPIE BiOS, 2024, San Francisco, California, United States

Conference Poster

Abstract

Mitochondria are vital organelles responsible for energy production and they undergo dynamic morphological changes influenced by the cell’s metabolic state. Mitochondrial membrane potential is an essential component in the process of energy storage in the cells. The membrane potential plays a crucial role in the functioning of mitochondria, and it can influence mitochondrial velocity through various mechanisms. Here we report correlation studies on the mitochondrial dynamics and mitochondrial membrane potential in mammalian cells using our home-built sMx-SPIM imaging system. The mitochondria are tracked manually by a self-developed algorithm to find the speed. The speed of mitochondria was determined by targeting mitochondria stained with MitoTracker Deep Red CMXRos dye and measurement of mitochondrial membrane potential was done by tetramethylrhodamine methyl ester (TMRM). Experimental studies were conducted on mammalian cells in alive conditions, and results are provided.

Citation Download Citation

Aiswarya K.S., Nikhil Dev Narendradev, Piyao Narumbam, Parul Jain, Srinivasa Murty Srinivasula, and Mayanglambam Suheshkumar Singh "A study on mitochondrial dynamics and membrane potential in mammalian cells using home-built simultaneous multiple-level magnification selective plane illumination microscopy (sMx-SPIM)", Proc. SPIE 12846, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXII, 128460E (12 March 2024); https://doi.org/10.1117/12.3003659

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available