Phase from defocus: transport of intensity or in-line holography?

Mikołaj Rogalski; Piotr Arcab; Emilia Wdowiak; Michał Józwik; Maciej Trusiak

doi:10.1117/12.3008453

12 March 2024 Phase from defocus: transport of intensity or in-line holography?

Mikołaj Rogalski, Piotr Arcab, Emilia Wdowiak, Michał Józwik, Maciej Trusiak

Proceedings Volume 12852, Quantitative Phase Imaging X; 128520L (2024) https://doi.org/10.1117/12.3008453
Event: SPIE BiOS, 2024, San Francisco, California, United States

Conference Poster

Abstract

Quantitative phase imaging techniques (QPI) enable to observe transparent samples with high contrast and with quantitative information about their optical thickness. Among the vast family of QPI methods, two of them: (1) transport of intensity equation (TIE) and (2) digital in-line holographic microscopy (DIHM), rely on retrieving phase from several images collected with different defocus. In this work, we preliminarily investigate, mainly numerically under simulated conditions, the differences between those two methods, showing that TIE is more suitable for imaging lower-frequency objects with small defocus distance difference between collected images (around several micrometers). On the other hand, DIHM performs better for higher frequency objects and images collected with larger defocus distance difference (hundreds of micrometers). Moreover, our results show that DIHM achieves better results, when all collected images are relatively far from focal plane (in the millimeter range rather than in micrometer range), while for TIE this parameter does not have a significant influence onto the retrieved phase.

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Mikołaj Rogalski, Piotr Arcab, Emilia Wdowiak, Michał Józwik, and Maciej Trusiak "Phase from defocus: transport of intensity or in-line holography?", Proc. SPIE 12852, Quantitative Phase Imaging X, 128520L (12 March 2024); https://doi.org/10.1117/12.3008453

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