Resolution-enhanced single-pixel fluorescence microscopy

L. Ordóñez; A. J. M. Lenz; N. Yoneda; M. Kumar; J. Lancis; O. Matoba; E. Tajahuerce

doi:10.1117/12.3017113

18 June 2024 Resolution-enhanced single-pixel fluorescence microscopy

L. Ordóñez, A. J. M. Lenz, N. Yoneda, M. Kumar, J. Lancis, O. Matoba, E. Tajahuerce

Proceedings Volume 12996, Unconventional Optical Imaging IV; 129960N (2024) https://doi.org/10.1117/12.3017113
Event: SPIE Photonics Europe, 2024, Strasbourg, France

Abstract

A novel single-pixel fluorescence microscope with super-resolution, based on structured illumination microscopy, has been developed to release the full potential of single-pixel microscopy. By employing enhanced spatially resolved patterns, the proposed microscope measures light intensities using a bucket detector and improves lateral resolution for both brightfield and fluorescence imaging by extending the spatial frequency spectrum. The results demonstrate significant improvements in lateral resolution, highlighting its application in fluorescence microscopy.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

L. Ordóñez, A. J. M. Lenz, N. Yoneda, M. Kumar, J. Lancis, O. Matoba, and E. Tajahuerce "Resolution-enhanced single-pixel fluorescence microscopy", Proc. SPIE 12996, Unconventional Optical Imaging IV, 129960N (18 June 2024); https://doi.org/10.1117/12.3017113

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available