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Clinical evidence underscores the intricate interplay between the heart and kidneys, where dysfunction in one organ contributes to progressive failure of both. In our previous work, using vibrational spectroscopy techniques, we demonstrated molecular changes in cardiac tissue post-uninephrectomy (UNX) and ischemia-reperfusion (I/R) surgeries in a rat cardiorenal syndrome (CS) model. It is now imperative to investigate whether structural changes in renal tissue following these two interventions are detectable. Vibrational spectroscopy serves as a powerful analytical technique with a fundamental role in molecular structure analysis. This method provides valuable insights into the intricate architecture of biomolecules, including proteins, lipids, and carbohydrates. Fourier-transform infrared (FTIR) microspectroscopy, in particular, emerges as a potent method for highresolution chemical imaging of various biological tissues, facilitating the analysis of molecular signatures indicative of physiological or pathological states. Despite the recognized utility of FTIR in various biomedical applications, its potential in assessing cardiorenal diseaseinduced lesions in heart and kidney tissues remains underexplored. Therefore, this study aims to bridge this gap by applying FTIR-imaging to identify spectroscopic markers related to renal complications. By characterizing the molecular fingerprints associated with pathological alterations in kidney tissue, this study aims to contribute to the development of non-invasive diagnostic tool for early detection and monitoring of renal dysfunction following surgical interventions. The findings of this study hold promise in advancing our understanding of the molecular mechanisms underlying renal complications, thereby facilitating timely interventions and the development of personalized therapeutic strategies in clinical settings.
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