Fluorescence enhancement of fluorophores in live cells using metallic nanoparticles

Marco Locarno; Qiangrui Dong; Xin Meng; Cristiano Glessi; Daan Brinks

doi:10.1117/12.3017177

20 June 2024 Fluorescence enhancement of fluorophores in live cells using metallic nanoparticles

Marco Locarno, Qiangrui Dong, Xin Meng, Cristiano Glessi, Daan Brinks

Proceedings Volume 13007, Neurophotonics II; 1300705 (2024) https://doi.org/10.1117/12.3017177
Event: SPIE Photonics Europe, 2024, Strasbourg, France

Conference Poster

Abstract

Plasmonic enhancement of fluorescence has been challenging in in vivo imaging applications. We present a study demonstrating the plasmonic enhancement of fluorescent membrane proteins within their native physiological environment using tailored metallic nanoparticles. This work highlights two schemes to influence the distance between the emitting dipoles and the enhancing nanoparticles, namely the addition of nanoparticles in the buffer solution and the incorporation in the polymer matrix at the bottom of the cells. Incorporating biological structures native to the cellular environment offers opportunities for the optimization of in vivo fluorescence imaging methods and the detection of membrane proteins.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Marco Locarno, Qiangrui Dong, Xin Meng, Cristiano Glessi, and Daan Brinks "Fluorescence enhancement of fluorophores in live cells using metallic nanoparticles", Proc. SPIE 13007, Neurophotonics II, 1300705 (20 June 2024); https://doi.org/10.1117/12.3017177

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