Automation of optical tweezers: an enabler for single cell analysis and diagnostic

Pedro Jorge; Joana Teixeira; Vicente Rocha; José Ribeiro; Nuno Silva

doi:10.1117/12.3022229

20 June 2024 Automation of optical tweezers: an enabler for single cell analysis and diagnostic

Pedro Jorge, Joana Teixeira, Vicente Rocha, José Ribeiro, Nuno Silva

Proceedings Volume 13008, Biophotonics in Point-of-Care III; 130080E (2024) https://doi.org/10.1117/12.3022229
Event: SPIE Photonics Europe, 2024, Strasbourg, France

Abstract

Sensing at the single cell level can provide insights into its dynamics and heterogeneity, yielding information otherwise unattainable with traditional biological methods where average population behavior is observed. In this context, optical tweezers provide the ability to select, separate, manipulate and identify single cells or other types of microparticles, potentially enabling single cell diagnostics. Forward or backscatter analysis of the light interacting with the trapped cells can provide valuable insights on the cell optical, geometrical and mechanical properties. In particular, the combination of tweezers systems with advanced machine learning algorithms can enable single cell identification capabilities. However, typical processing pipelines require a training stage which often struggles when trying to generalize to new sets of data. In this context, fully automated tweezers system can provide mechanisms to obtain much larger datasets with minimum effort form the users, while eliminating procedural variability. In this work, a pipeline for full automation of optical tweezers systems is discussed. A performance comparison between manually operated and fully automated tweezers systems is presented, clearly showing advantages of the latter. A case study demonstrating the ability of the system to discriminate molecular binding events on microparticles is presented.

Conference Presentation

Citation Download Citation

Pedro Jorge, Joana Teixeira, Vicente Rocha, José Ribeiro, and Nuno Silva "Automation of optical tweezers: an enabler for single cell analysis and diagnostic", Proc. SPIE 13008, Biophotonics in Point-of-Care III, 130080E (20 June 2024); https://doi.org/10.1117/12.3022229

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available