The present work shows design, development, and testing of the multimodal optical setup for simultaneous auto-fluorescence imaging, spectroscopy and quantitative phase microscopy (SAF-QPM) from same field of view (FOV). The SAF-QPM combines the capabilities of autofluorescence, offering molecular insights into metabolic activities, with quantitative phase imaging, providing nanometres-level sensitivity to cellular morphology and refractive index distribution. The autofluorescence serves as a biomarker for abnormal cellular changes associated with cancer development. Simultaneous incorporation of quantitative phase microscopy of the same tissue section probes the refractive index-dependent changes in the cellular morphology associated with cancer development. Non-interferometric QPI technique is used to retrieve the phase information of the sample, which overcomes the limitations of instability and coherent noise associated with interferometric QPI techniques. The incorporation of simultaneous AF and QPM from the same field of view enhances the accuracy and specificity of label-free cancer diagnosis.
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