This study investigates the development of a blood-mimicking fluid for applications in optical imaging and characterization. Intralipid, a scatterer commonly used to mimic biological tissue, is explored for its ability to replicate the optical properties of blood. Diffuse reflectance spectroscopy, a technique measuring reflected light was employed to analyze intralipid solutions at varying concentrations (1% - 10%). Concurrently, Monte Carlo simulations were conducted to model light scattering behavior within the intralipid. The findings suggest that intralipid concentrations can be adjusted to mimic the diffused reflected light signature of either oxygenated or deoxygenated blood at the chosen wavelengths. This signifies the potential of intralipid as a substitute for blood in various optical studies, offering a valuable tool for characterizing biological tissues in spectroscopy and imaging applications.
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