Research on high throughput automated pipette workstation

Luyang Duanmu; Yang Lv; Yuanhua Yu

doi:10.1117/12.3036888

13 July 2024 Research on high throughput automated pipette workstation

Luyang Duanmu, Yang Lv, Yuanhua Yu

Proceedings Volume 13208, Third International Conference on Biomedical and Intelligent Systems (IC-BIS 2024); 132080B (2024) https://doi.org/10.1117/12.3036888
Event: 3rd International Conference on Biomedical and Intelligent Systems (IC-BIS 2024), 2024, Nanchang, China

Abstract

With the progress of molecular diagnostic technologies, manual nucleic acid extraction methods no longer suffice to meet current demands. In order to enhance the positioning precision and liquid handling accuracy of a 25-well high-throughput automated pipetting workstation, we investigated an S-shaped acceleration-deceleration algorithm based on the Sigmoid function for controlling stepper motors along the X, Y, and Z axes. Subsequently, we constructed a corresponding mechanical device. Through this apparatus, we conducted experiments to assess positioning precision, liquid handling accuracy, and biological performance. The experimental results revealed that the absolute error in positioning precision was less than 0.1 mm, and the coefficient of variation (CV) for liquid handling accuracy was below 1%. Furthermore, post-purification nucleic acid A260/A280 ratios were measured at approximately 1.911, falling within the range of 1.8 to 2.0. Our devised system further improved both positioning and liquid handling precision, thereby advancing high-throughput automated nucleic acid extraction technology.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Luyang Duanmu, Yang Lv, and Yuanhua Yu "Research on high throughput automated pipette workstation", Proc. SPIE 13208, Third International Conference on Biomedical and Intelligent Systems (IC-BIS 2024), 132080B (13 July 2024); https://doi.org/10.1117/12.3036888

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KEYWORDS

Nucleic acids

Liquids

Magnetism

Diagnostics

Pulse signals

Design

Lead

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