Innovative two-photon technology reveals novel Biological phenomena

Shuai Wang; Shiqi Wang; Yueshu Feng; Jiaqing Guo; Liwei Liu; Shuai Ye

doi:10.1117/12.3036844

13 July 2024 Innovative two-photon technology reveals novel Biological phenomena

Shuai Wang, Shiqi Wang, Yueshu Feng, Jiaqing Guo, Liwei Liu, Shuai Ye

Proceedings Volume 13208, Third International Conference on Biomedical and Intelligent Systems (IC-BIS 2024); 132080E (2024) https://doi.org/10.1117/12.3036844
Event: 3rd International Conference on Biomedical and Intelligent Systems (IC-BIS 2024), 2024, Nanchang, China

Abstract

Two-photon microscopy plays a significant role in biological applications, as it enables non-invasive, high-resolution imaging of thick tissue samples. This paper begins by elucidating fundamental concepts of two-photon microscopy to facilitate a deeper understanding of its luminescent mechanism. Subsequently, we provide a comprehensive overview of the imaging system, encompassing the light source, scanning unit, fluorescence collection, and detection components. As two-photon microscopy continues to evolve, We undertake assessments of cutting-edge optical instrumentation, emphasizing novel approaches for imaging across wider fields of view, at higher speeds, and with increased volumetric imaging capabilities. Furthermore, we summarize and compare the technical specifications of advanced two-photon imaging. Following this, the manuscript showcases the transformative applications of these innovative technologies in biological imaging, providing further evidence of the indispensable role of two-photon microscopy in biomedical imaging research.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Shuai Wang, Shiqi Wang, Yueshu Feng, Jiaqing Guo, Liwei Liu, and Shuai Ye "Innovative two-photon technology reveals novel Biological phenomena", Proc. SPIE 13208, Third International Conference on Biomedical and Intelligent Systems (IC-BIS 2024), 132080E (13 July 2024); https://doi.org/10.1117/12.3036844

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