Nile Blue derivatives as lysosomotropic photosensitizers

Chi-Wei Lin; Janine R. Shulok; S. D. Kirley; Louis Cincotta; James W. Foley

doi:10.1117/12.44061

1 June 1991 Nile Blue derivatives as lysosomotropic photosensitizers

Chi-Wei Lin, Janine R. Shulok, S. D. Kirley, Louis Cincotta, James W. Foley

Proceedings Volume 1426, Optical Methods for Tumor Treatment and Early Diagnosis: Mechanisms and Techniques; (1991) https://doi.org/10.1117/12.44061
Event: Optics, Electro-Optics, and Laser Applications in Science and Engineering, 1991, Los Angeles, CA, United States

Abstract

The benzophenoxazines, including several Nile blue analogues, are a unique group of dyes that localize selectively in animal tumors. Chemical modifications of Nile blue A can yield derivatives with high 1O2 quantum yields. These derivatives represent a group of potentially effective photosensitizers for selective phototherapy of malignant tumors. In vitro evaluation of these derivatives has indicated that those with high 1O2 yields are very effective in mediating the photocytotoxicity of tumor cells. This photodynamic effect is most likely mediated through the action of 1O2, since photoirradiation under D2O enhanced and under hypoxic conditions diminished the photocytotoxic action. The subcellular localization of these photosensitizers in bladder tumor cells in culture was examined by light and fluorescence microscopies as well as by histochemical and biochemical studies. The results indicate that these dyes are localized primarily in the lysosome. The cellular uptake and retention of these dyes is energy- and pH-dependent. Agents such as nigericin, which alter the transmembrane pH gradient, reduced uptake and enhanced efflux of the dyes, while agents such as valinomycin, which reduce cellular membrane potential, had no effect on the uptake. These findings are consistent with having ion-trapping as the mechanism for the uptake of these dyes. Photoirradiation of sensitizer-treated cells obliterated lysosomes in a light-dose and drug-dose dependent fashion. Release of the hydrolytic enzymes may be the main cause for subsequent cell death since the cytolytic effect was reduced by a specific inhibitor of lysosomal proteolytic enzyme. A lysosomotropic photosensitization mechanism is therefore proposed for the photocytotoxic action of the Nile blue derivatives. This mechanism may provide an approach to the development of new photosensitizers for the effective and selective destruction of malignant tumors.

Citation Download Citation

Chi-Wei Lin, Janine R. Shulok, S. D. Kirley, Louis Cincotta, and James W. Foley "Nile Blue derivatives as lysosomotropic photosensitizers", Proc. SPIE 1426, Optical Methods for Tumor Treatment and Early Diagnosis: Mechanisms and Techniques, (1 June 1991); https://doi.org/10.1117/12.44061

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