Microscopic feature extraction from optical sections of contracting cardiac muscle cells recorded at high speed

Kenneth P. Roos; David S. Lake; Bradford A. Lubell

doi:10.1117/12.44138

1 May 1991 Microscopic feature extraction from optical sections of contracting cardiac muscle cells recorded at high speed

Kenneth P. Roos, David S. Lake, Bradford A. Lubell

Author Affiliations +

Proceedings Volume 1428, Three-Dimensional Bioimaging Systems and Lasers in the Neurosciences; (1991) https://doi.org/10.1117/12.44138
Event: Optics, Electro-Optics, and Laser Applications in Science and Engineering, 1991, Los Angeles, CA, United States

Abstract

The rapid motion of microscopic features such as the cross-striations of contracting cardiac muscle cells are difficult to capture with conventional RS-170 video systems and image processing approaches. In this report, efforts to extract, enhance and analyze striation data from widefield optical sections of single contracting cells recorded with a charge-coupled device (CCD) video camera modified for high-speed RS-170 compatible operation are described. Each video field from the camera provides four 1/4 height images separated by 4 ms in time for a 240 Hz image acquisition rate. Data are continuously recorded on S-VHS video tape during each experiment. Selected image sequences are digitized field by field and stored in a computer system under automated software control. The four individual images in each video field are separated, geometrically corrected for time base error, and reassembled as a single sequence of images for interpretable visualization. The images are then processed with digital filters and gray scale expansion to preferentially enhance the cross-striations and minimize out of focus features. Regions within each image containing striations are identified and their positions determined and followed during the contraction cycle to obtain individual, regional and cellular sarcomere dynamics. This approach permits the critical evaluation of the magnitude, time course and uniformity of contractile function throughout the volume of a single cell with higher temporal and spatial resolutions than previously possible.

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Kenneth P. Roos, David S. Lake, and Bradford A. Lubell "Microscopic feature extraction from optical sections of contracting cardiac muscle cells recorded at high speed", Proc. SPIE 1428, Three-Dimensional Bioimaging Systems and Lasers in the Neurosciences, (1 May 1991); https://doi.org/10.1117/12.44138

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KEYWORDS

Video

Image processing

Image filtering

3D image processing

Cameras

Digital filtering

Heart

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