Imaging of tumors by time-delayed laser-induced fluorescence

M. Kohl; Joerg Neukammer; Uwe Sukowski; Herbert H. Rinneberg; Hanns-joerg Sinn; E. A. Friedrich; Georgi Graschew; Peter M. Schlag; Dieter Woehrle

doi:10.1117/12.48208

1 November 1991 Imaging of tumors by time-delayed laser-induced fluorescence

M. Kohl, Joerg Neukammer, Uwe Sukowski, Herbert H. Rinneberg, Hanns-joerg Sinn, E. A. Friedrich, Georgi Graschew, Peter M. Schlag, Dieter Woehrle

Author Affiliations +

Proceedings Volume 1525, Future Trends in Biomedical Applications of Lasers; (1991) https://doi.org/10.1117/12.48208
Event: MedTech '91, 1991, Berlin, Germany

Abstract

A technique to improve signal-to-background ratio in fluorescence images of superficially growing tumors marked with photosensitizers is described. Time-delayed detection of fluorescence following pulsed-laser excitation allows suppression of the autofluorescence background falling into the fluorescence band of the photosensitizer. This technique exploits the longer fluorescence-decay times of porphyrin-based photosensitizers compared to average decay times of tissue autofluorescence. The feasibility of time-delayed fluorescence imaging of tumors has been demonstrated in vitro. From time-delayed fluorescence spectra the authors infer that the ratio between photosensitizer signal and autofluorescence background can be improved by about one order of magnitude.

Citation Download Citation

M. Kohl, Joerg Neukammer, Uwe Sukowski, Herbert H. Rinneberg, Hanns-joerg Sinn, E. A. Friedrich, Georgi Graschew, Peter M. Schlag, and Dieter Woehrle "Imaging of tumors by time-delayed laser-induced fluorescence", Proc. SPIE 1525, Future Trends in Biomedical Applications of Lasers, (1 November 1991); https://doi.org/10.1117/12.48208

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