New method for tissue indentification: resonance fluorescence spectroscopy

Walter Neu

doi:10.1117/12.48212

1 November 1991 New method for tissue indentification: resonance fluorescence spectroscopy

Walter Neu

Proceedings Volume 1525, Future Trends in Biomedical Applications of Lasers; (1991) https://doi.org/10.1117/12.48212
Event: MedTech '91, 1991, Berlin, Germany

Abstract

The method proposed in this paper is based on the detection of resonantly enhanced fluorescence emission induced by a tunable dye laser. First test on anorganic samples exposed to air and to saline solution demonstrate the potential of this technique. A XeCl excimer-laser ((lambda) equals308 nm) pulse, guided by quartz fibers, causes an efficient ablation of the irradiated samples. The specific species to be detected in the ablation plume determines the wavelength of the narrow-band dye-laser radiation. Preferably, it is set to a strong transition of the selected ablation product. Taking into account the formation of the plume, the dye-laser pulse is applied with a certain delay in order to excite resonantly the chosen species in the plume. The resulting resonance fluorescence is then guided by optical fibers to an OMA system. Compared to the broad-band excimer-laser-indiced fluorescence during the ablation process, the resonance fluorescence signal shows a distinct and easily detectable sharp peak. The signal-to-background ratio is improved by one order of magnitude. The achieved increase in sensitivity as well as selectivity is for the benefit of a reliable identification of ablated tissue.

Citation Download Citation

Walter Neu "New method for tissue indentification: resonance fluorescence spectroscopy", Proc. SPIE 1525, Future Trends in Biomedical Applications of Lasers, (1 November 1991); https://doi.org/10.1117/12.48212

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