Site-specific laser modification (cleavage) of oligodeoxynucleotides

L. Z. Benimetskaya; N. V. Bulychev; Andrew L. Kozionov; A. A. Koshkin; Andrey V. Lebedev; S. Yu. Novozhilov; Mark I. Stockman

doi:10.1117/12.48221

1 November 1991 Site-specific laser modification (cleavage) of oligodeoxynucleotides

L. Z. Benimetskaya, N. V. Bulychev, Andrew L. Kozionov, A. A. Koshkin, Andrey V. Lebedev, S. Yu. Novozhilov, Mark I. Stockman

Author Affiliations +

Proceedings Volume 1525, Future Trends in Biomedical Applications of Lasers; (1991) https://doi.org/10.1117/12.48221
Event: MedTech '91, 1991, Berlin, Germany

Abstract

Sequence-specific nonlinear photomodification of a ''target'' oligodeoxynucleotide pAGAGTATTGACTTA (14-mer) has been carried out with the aid of a complementary fluorescent probe, consisting of oligodeoxynucleotide pAATACTCT and a chromophore group (EtBr) attached to its 5-ft end. An approach is used which is a combination of the methods of two-quantum sensitized excitation and oligonucleotide-directed modification. It is based on the following: A chromophore is covalently linked to the probe oligonucleotide which is complementary to a given region of nucleic acid (NA). Irradiation is carried out with laser light which is not absorbed directly by NA but is quasiresonantly absorbed by the chromophore. At sufficiently high intensity of light stepwise excitation of the chromophore to a higher singlet state takes place, and radiativeless transfer of the excitation energy to the NA occurs within the radius of several angstroms around the chromophore. The transferred energy corresponds to the far-UV region and it sufficient for induction of different modifications of NA, including phosphodiester-bond breakage.

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L. Z. Benimetskaya, N. V. Bulychev, Andrew L. Kozionov, A. A. Koshkin, Andrey V. Lebedev, S. Yu. Novozhilov, and Mark I. Stockman "Site-specific laser modification (cleavage) of oligodeoxynucleotides", Proc. SPIE 1525, Future Trends in Biomedical Applications of Lasers, (1 November 1991); https://doi.org/10.1117/12.48221

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