Synergism between photochemical and ionizing radiation effects in MCF-7 cells in vitro

Gulshan Ara; Terence S. Herman; Archana Varshney; Timothy Korbut; Beverly A. Teicher

doi:10.1117/12.137459

7 August 1992 Synergism between photochemical and ionizing radiation effects in MCF-7 cells in vitro

Gulshan Ara, Terence S. Herman, Archana Varshney, Timothy Korbut, Beverly A. Teicher

Proceedings Volume 1646, Laser-Tissue Interaction III; (1992) https://doi.org/10.1117/12.137459
Event: OE/LASE '92, 1992, Los Angeles, CA, United States

Abstract

Both PtCl₄ (Rh-123)₂ and PtCl₄ (Nile Blue)₂ interact positively in trimodality therapy including each drug, x-ray, and light (400 - 800 nm) in killing human mammary carcinoma cells (MCF-7) in vitro. The combination treatment results in more than additive killing as assessed by product of the surviving fractions and a significant reduction of the shoulder of the x-ray survival curve. Both of the drugs participate in photodynamic therapy (PDT) in drug and light dose dependent manner. The primary subcellular targets for the neutral platinum complexes were indicated to be the nuclear DNA as opposed to the mitochondria for Rh-123 or the lysosomes for Nile Blue. Because the nuclear DNA is also the primary target for ionizing radiation, drug plus light plus x-ray might cause supraadditive killing. Both PtCl₄ (Rh-123)₂ and PtCl₄ (Nile Blue)₂ have been reported to be relatively non-toxic in vivo compared to the anionic or cationic compounds currently being used in photodynamic therapy. Based on these results PtCl₄ (Rh-123)₂ and PtCl₄ (Nile Blue)₂ have the potential for use in photodynamic therapy and in trimodality therapy.

Citation Download Citation

Gulshan Ara, Terence S. Herman, Archana Varshney, Timothy Korbut, and Beverly A. Teicher "Synergism between photochemical and ionizing radiation effects in MCF-7 cells in vitro", Proc. SPIE 1646, Laser-Tissue Interaction III, (7 August 1992); https://doi.org/10.1117/12.137459

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