Measuring oxygen pressures using triplet quenching of Pd-porphine

Michiel Sinaasappel; C. Ince; E. A. Sanderse; Hajo A. Bruining

doi:10.1117/12.167420

1 February 1994 Measuring oxygen pressures using triplet quenching of Pd-porphine

Michiel Sinaasappel, C. Ince, E. A. Sanderse, Hajo A. Bruining

Proceedings Volume 2083, Microscopy, Holography, and Interferometry in Biomedicine; (1994) https://doi.org/10.1117/12.167420
Event: Europto Biomedical Optics '93, 1993, Budapest, Hungary

Abstract

A non-invasive optical method for measuring free oxygen in vivo is described. The method, introduced by Wilson and co-workers, is based on the quenching of the triplet state of Pd- porphine by oxygen and is described by the Stern-Volmer relation. The quenching of the triplet state is determined by measurement of the phosphorescence decay following excitation by a pulse of light. Measuring decay times has the advantage that they are independent of the changing optical properties of tissue and concentration of the dye. We describe a setup to measure the phosphorescence decay and present the values of K_q and (tau) _o as a function of pH and temperature. Furthermore, some experiments on a rat liver and gut are presented.

Citation Download Citation

Michiel Sinaasappel, C. Ince, E. A. Sanderse, and Hajo A. Bruining "Measuring oxygen pressures using triplet quenching of Pd-porphine", Proc. SPIE 2083, Microscopy, Holography, and Interferometry in Biomedicine, (1 February 1994); https://doi.org/10.1117/12.167420

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