Matrix metalloproteinase expression in excimer laser wounded rabbit corneas

Taewon Hahn; Wallace Chamon; Yonja Akova; Walter J. Stark M.D.; William G. Stetler-Stevenson; Dimitri T. Azar M.D.

doi:10.1117/12.178583

23 June 1994 Matrix metalloproteinase expression in excimer laser wounded rabbit corneas

Taewon Hahn, Wallace Chamon, Yonja Akova, Walter J. Stark M.D., William G. Stetler-Stevenson, Dimitri T. Azar M.D.

Proceedings Volume 2126, Ophthalmic Technologies IV; (1994) https://doi.org/10.1117/12.178583
Event: OE/LASE '94, 1994, Los Angeles, CA, United States

Abstract

This study was performed to obtain information about matrix metalloproteinase (MMP) expression in excimer-wounded corneas and to determine whether MMPs expression correlates with the depth of the ablation. 6-mm excimer keratectomy (60 or 180 micrometers ) was performed using the 193-mm ArF excimer laser on 12 NZW rabbits. Corneas treated with mechanical epithelial debridement and untreated corneas served as controls. Rabbits were killed at 20 and 30 hr after laser ablation. Zymography after SDS extraction was performed on regenerated central epithelium and the central stroma to determine MMPs expression. We observed enzymatic activity of a 92 KDa band in the epithelium of excimer-ablated corneas but not in that following debridement wounds and untreated controls. The expression of the 92 KDa MMP was most pronounced with the deeper excimer ablation. A 72 KDa band of enzymatic activity present in the stroma of all treated and control eyes was also seen in the epithelium of excimer-ablated corneas. These proteolytic enzymes may play an important role in wound healing and remodelling after excimer keratectomy.

Citation Download Citation

Taewon Hahn, Wallace Chamon, Yonja Akova, Walter J. Stark M.D., William G. Stetler-Stevenson, and Dimitri T. Azar M.D. "Matrix metalloproteinase expression in excimer laser wounded rabbit corneas", Proc. SPIE 2126, Ophthalmic Technologies IV, (23 June 1994); https://doi.org/10.1117/12.178583

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