Biochemical sensing in tissues: determination of fluorescent lifetimes in multiply scattering media using frequency-domain spectroscopy

Christina L. Burch; Joseph R. Lakowicz; Eva Marie Sevick-Muraca

doi:10.1117/12.176003

19 May 1994 Biochemical sensing in tissues: determination of fluorescent lifetimes in multiply scattering media using frequency-domain spectroscopy

Christina L. Burch, Joseph R. Lakowicz, Eva Marie Sevick-Muraca

Author Affiliations +

Proceedings Volume 2135, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases; (1994) https://doi.org/10.1117/12.176003
Event: OE/LASE '94, 1994, Los Angeles, CA, United States

Abstract

Numerical solutions to the diffusion equation describing light propagation in tissues are presented. The results show that time- dependent measurements of light propagation can be used to monitor the fluorescent lifetimes of a fluorophore uniformly distrusted in tissues. With proper referencing, frequency-domain measurements of phase-shift, (Theta) , may allow quantitation of fluorescent lifetimes, (tau) , independent of changes in the local absorption and scattering properties. These results point to a new approach for noninvasive diagnostic monitoring through quantitation of fluorescent lifetimes, (tau) .

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Christina L. Burch, Joseph R. Lakowicz, and Eva Marie Sevick-Muraca "Biochemical sensing in tissues: determination of fluorescent lifetimes in multiply scattering media using frequency-domain spectroscopy", Proc. SPIE 2135, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases, (19 May 1994); https://doi.org/10.1117/12.176003

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