Cell patterns for biosensors manufactured with laser tweezers

Joachim Renken; Stefan Seeger

doi:10.1117/12.229505

8 January 1996 Cell patterns for biosensors manufactured with laser tweezers

Joachim Renken, Stefan Seeger

Proceedings Volume 2629, Biomedical Optoelectronics in Clinical Chemistry and Biotechnology; (1996) https://doi.org/10.1117/12.229505
Event: BiOS Europe '95, 1995, Barcelona, Spain

Abstract

A drug screening assay based on patterned cells was developed. The patterning was performed by detaching single lymphocytes with laser tweezers from a poly-ethylene oxide (PEO) surface and immobilizing them on a surface coated with Cell-Tak^R, a strong cellular adhesive. The detachment force of the cells from the PEO-surface was determined on a single cell base with the laser tweezers to be between 1.5 and 4.5 pN. Different lymphocyte classes were loaded with the fluorescent calcium indicator Fluo-3 and the pH indicator BCECF. Specific stimulation of the immobilized cells is monitored.

Citation Download Citation

Joachim Renken and Stefan Seeger "Cell patterns for biosensors manufactured with laser tweezers", Proc. SPIE 2629, Biomedical Optoelectronics in Clinical Chemistry and Biotechnology, (8 January 1996); https://doi.org/10.1117/12.229505

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