Compact microscope-based 850-nm optical tweezers

Carlo Frediani; S. Grego; L. Guidoni; Ennio Arimondo

doi:10.1117/12.229502

8 January 1996 Compact microscope-based 850-nm optical tweezers

Carlo Frediani, S. Grego, L. Guidoni, Ennio Arimondo

Proceedings Volume 2629, Biomedical Optoelectronics in Clinical Chemistry and Biotechnology; (1996) https://doi.org/10.1117/12.229502
Event: BiOS Europe '95, 1995, Barcelona, Spain

Abstract

Emission from a single mode 100 mW laser diode at 850 nm is used for realizing optical tweezers: the laser beam is introduced into a microscope and focused by the objective into the object plane. Injection of the beam into a 40X microscope objective has been studied and the position and the size of the waist measured. The trap performance was studied as a function of the dimensions of the trapped particles. Trapping of polystyrene latex spheres of different size (from 0.2 micrometer to 6 micrometer) was observed in different conditions of laser power and transverse velocity of the spheres. Biological objects, Tetraselmis, of large dimension (around 10 micrometer) were also studied. We demonstrate the existence of an optimal range of size of the particles to be trapped. Furthermore we measure minimum trapping power required for trapping and the maximum speed of the trapped objects as a function of the dimensions.

Citation Download Citation

Carlo Frediani, S. Grego, L. Guidoni, and Ennio Arimondo "Compact microscope-based 850-nm optical tweezers", Proc. SPIE 2629, Biomedical Optoelectronics in Clinical Chemistry and Biotechnology, (8 January 1996); https://doi.org/10.1117/12.229502

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