Optimization of fluorimetric lipid membrane biosensor sensitivity through manipulation of membrane structure and nitrobenzoxadiazole dipalmitoylphosphatidylethanolamine concentration

Jason D. A. Shrive; Ulrich J. Krull

doi:10.1117/12.229189

3 January 1995 Optimization of fluorimetric lipid membrane biosensor sensitivity through manipulation of membrane structure and nitrobenzoxadiazole dipalmitoylphosphatidylethanolamine concentration

Jason D. A. Shrive, Ulrich J. Krull

Author Affiliations +

Proceedings Volume 2631, Medical and Fiber Optic Sensors and Delivery Systems; (1995) https://doi.org/10.1117/12.229189
Event: BiOS Europe '95, 1995, Barcelona, Spain

Abstract

In the work reported here, surface concentrations of 0.027 and 0.073 molecules nm^-2 of the fluorescent membrane probe molecule nitrobenzoxadiazole dipalmitoylphosphatidylethanolamine (NBD-PE) were shown to yield optimum sensitivity for fluorimetric transduction of membrane structural perturbations for lipid membrane-based biosensor development. These optima were obtained through correlation of experimental data with theoretical predictions of optimum surface concentrations based on a model for NBD-PE self quenching previously published by our group. It was also determined that membrane structural heterogeneity improves the sensitivity of NBD-PE labeled membrane transducers. Together with fluorescence microscopy, observations of surface potential change upon compression or expansion of phosphatidylcholine (PC)/phosphatidic acid (PA) monolayers were used to qualitatively indicate the degree of structural heterogeneity in these membranes. It was determined that sub-microscopic domains must exist in microscopically homogeneous egg PC/egg PA membranes in order to facilitate the observed NBD-PE self-quenching responses upon alteration of bulk pH and therefore, membrane surface electrostatics and structure.

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Jason D. A. Shrive and Ulrich J. Krull "Optimization of fluorimetric lipid membrane biosensor sensitivity through manipulation of membrane structure and nitrobenzoxadiazole dipalmitoylphosphatidylethanolamine concentration", Proc. SPIE 2631, Medical and Fiber Optic Sensors and Delivery Systems, (3 January 1995); https://doi.org/10.1117/12.229189

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