Application of avalanche photodiodes to confocal and confocal florescence microscopy

Thomas G. Brown; Stephen T. Kreger

doi:10.1117/12.237495

10 April 1996 Application of avalanche photodiodes to confocal and confocal florescence microscopy

Thomas G. Brown, Stephen T. Kreger

Proceedings Volume 2655, Three-Dimensional Microscopy: Image Acquisition and Processing III; (1996) https://doi.org/10.1117/12.237495
Event: Electronic Imaging: Science and Technology, 1996, San Jose, CA, United States

Abstract

We describe the use of avalanche photodiodes as solid state photon counters in scanning confocal microscopy. Photon counters have been of limited use for moderate-to-rapid image acquisition speeds due to comparatively low saturation count rates. Several approaches offer the promise of real-time photon-limited image acquisition using off-the-shelf components and dark count rates which are fully adequate for imaging. We characterize in some detail a gated NPN switch/pulse-bias circuit which offers excellent, economical performance over a wide wavelength range, and discuss specific imaging applications.

Citation Download Citation

Thomas G. Brown and Stephen T. Kreger "Application of avalanche photodiodes to confocal and confocal florescence microscopy", Proc. SPIE 2655, Three-Dimensional Microscopy: Image Acquisition and Processing III, (10 April 1996); https://doi.org/10.1117/12.237495

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