Laser time-resolved spectroscopy of spectral and spatial heterogeneity of fluorescent probe molecules in model membranes

Yu. V. Zvinevich

doi:10.1117/12.2316037

1 September 1996 Laser time-resolved spectroscopy of spectral and spatial heterogeneity of fluorescent probe molecules in model membranes

Yu. V. Zvinevich

Author Affiliations +

Proceedings Volume 2778, 17th Congress of the International Commission for Optics: Optics for Science and New Technology; 27789O (1996) https://doi.org/10.1117/12.2316037
Event: 17th Congress of the International Commission for Optics: Optics for Science and New Technology, 1996, Taejon, Korea, Republic of

Abstract

The spectral and polarization time-resolved characteristics of the fluorescent probe 1-phenylnaphthylamine (1-AN) [1] in model and red blood cell (RBC) membranes using laser spectrofluorimeter have been studied. It is shown that the electronic spectra of probe in model membranes inhomogeneously broadened. Inhomogeneous broadening affect significantly the spectroscopic properties of probe in membranes, such as the time-dependent fluorescence Stokes shift, the dependence of the fluorescence instantaneous spectra and fluorescence kinetics on excitation wavelength.

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Yu. V. Zvinevich "Laser time-resolved spectroscopy of spectral and spatial heterogeneity of fluorescent probe molecules in model membranes", Proc. SPIE 2778, 17th Congress of the International Commission for Optics: Optics for Science and New Technology, 27789O (1 September 1996); https://doi.org/10.1117/12.2316037

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