Thionine as an indicator for use as a hydrogen sulfide optode

Oliver Kohls; Ingo Klimant; Gerhard A. Holst; Michael Kuehl

doi:10.1117/12.260606

10 December 1996 Thionine as an indicator for use as a hydrogen sulfide optode

Oliver Kohls, Ingo Klimant, Gerhard A. Holst, Michael Kuehl

Proceedings Volume 2836, Chemical, Biochemical, and Environmental Fiber Sensors VIII; (1996) https://doi.org/10.1117/12.260606
Event: SPIE's 1996 International Symposium on Optical Science, Engineering, and Instrumentation, 1996, Denver, CO, United States

Abstract

The amount of dissolved hydrogen sulfide is an important parameter in many environmental applications. Conventional methods for H₂S detection depend on iodometric titration or spectroscopic measurements. Unfortunately these methods are not applicable for direct measurements in natural systems. A recently described method for the on-line detection of H₂S is based on quenching of fluorescence of thioneine. The reaction between H₂S and thioneine was described as reversible photo-reduction. This reaction was tested in order to design an optical microsensor for the measurement of H₂S in sediments and other biological systems. We immobilized thioneine in several matrices and investigated these materials with respect to response time, mechanical stability, the influence of the excitation light and the reversibility. The sensing materials were deposited on the tip of optical fibers. The measuring system for the excitation and detection of the fluorescence consisted of a yellow light emitting diode, a fiber-optic coupler and a photomultiplier. The excitation light was intensity modulated to enable measurements in ambient light. Our results indicate that the thioneine based reaction scheme for H₂S detection is not very suitable for use in a H₂S optode due to lack of reversibility, long response times, and the need for regeneration of the sensor chemistry.

Citation Download Citation

Oliver Kohls, Ingo Klimant, Gerhard A. Holst, and Michael Kuehl "Thionine as an indicator for use as a hydrogen sulfide optode", Proc. SPIE 2836, Chemical, Biochemical, and Environmental Fiber Sensors VIII, (10 December 1996); https://doi.org/10.1117/12.260606

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