Microcavitation and cell injury in RPE cells following short-pulsed laser irradiation

Michael W. Kelly; Charles P. Lin

doi:10.1117/12.275473

16 June 1997 Microcavitation and cell injury in RPE cells following short-pulsed laser irradiation

Michael W. Kelly, Charles P. Lin

Proceedings Volume 2975, Laser-Tissue Interaction VIII; (1997) https://doi.org/10.1117/12.275473
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

Our research investigates the mechanism of retinal injury from short laser pulses. We used an ex-vivo porcine model and time resolved strobe imaging to examine mechanical effects associated with sub-nanosecond absorption by the retinal pigment epithelium (RPE). We present a unique method of imaging high speed event sin the RPE with a resolution of approximately 1 micrometers . Microcavitation bubbles were first imaged around single melanosomes, after irradiation with a 40 ps, 532 nm pulse. The threshold for bubble formation was 50 mJ/cm². A few times threshold, stress waves could be observed around the melanosomes. At 0.68 J/cm² we measured shock waves with an average velocity of 2700 m/s, 1 ns after irradiation. After exposures above 50 mJ/cm², RPE cells were found to be non-viable. We compare these results with MVL data collected using live animal models.

Citation Download Citation

Michael W. Kelly and Charles P. Lin "Microcavitation and cell injury in RPE cells following short-pulsed laser irradiation", Proc. SPIE 2975, Laser-Tissue Interaction VIII, (16 June 1997); https://doi.org/10.1117/12.275473

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