Fiber optic micro-optrodes for intracellular chemical measurements

Scott McCulloch; Deepak G. Uttamchandani

doi:10.1117/12.273521

7 May 1997 Fiber optic micro-optrodes for intracellular chemical measurements

Scott McCulloch, Deepak G. Uttamchandani

Proceedings Volume 2980, Advances in Fluorescence Sensing Technology III; (1997) https://doi.org/10.1117/12.273521
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

A simple, low cost technique is described for producing miniaturized fiber optic chemical sensors, and results obtained using these devices to monitor pH within the micro- chemical environment of individual mammalian cells (e.g. mouse embryonic fibroblasts) is reported. The technology is based on the use of submicron optical fiber tips which have fluorescent chemical or biological reagents immobilized on their tip surface. Fiber tips (0.1 - 1 micrometers ) were formed by drawing single-mode optical fiber (125 micrometers outer diameter) in a commercial fusion splicer. Fluorescent dye-doped sol- gel films and polymers were then deposited on the surface of the fiber tip using dip-coating techniques and photochemical synthesis respectively. Laser light from an argon ion laser was coupled into the fibers untapered end, and the fluorescence light equipped with a cooled photomultiplier detection system. The sensors that have been developed using these coating technologies are reversible and have response times of a few tenths of a second. We are currently expanding this sensing technique to monitor other biomedical parameters (e.g. oxygen concentration), by utilizing alternative chemical indicators that are compatible with the immobilization techniques described.

Citation Download Citation

Scott McCulloch and Deepak G. Uttamchandani "Fiber optic micro-optrodes for intracellular chemical measurements", Proc. SPIE 2980, Advances in Fluorescence Sensing Technology III, (7 May 1997); https://doi.org/10.1117/12.273521

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