Molecular recognition and optical detection of biological pathogens at biomimetic membrane interfaces

John J. Pan; Deborah H. Charych

doi:10.1117/12.267115

14 February 1997 Molecular recognition and optical detection of biological pathogens at biomimetic membrane interfaces

John J. Pan, Deborah H. Charych

Proceedings Volume 3040, Smart Structures and Materials 1997: Smart Materials Technologies; (1997) https://doi.org/10.1117/12.267115
Event: Smart Structures and Materials '97, 1997, San Diego, CA, United States

Abstract

Molecular recognition sites on cell membranes serve as the main communication channels between the inside of a cell and its surroundings. Upon receptor binding, cellular messages such as ion channel opening or activation of enzymes are triggered. In this report, we demonstrate that artificial cell membranes made from conjugated lipid polymers (polydiacetylene) can, on a simple level, mimic membrane processes of molecular recognition and signal transduction. The ganglioside, GMI was incorporated into polydiacetylene liposomes. Molecular recognition of cholera toxin at the interface of the liposome resulted in a change of the membrane color due to conformational changes in the conjugated (ene-yne) polymer backbone. The `colored liposomes' might be used as simple colorimetric sensors for drug screening or as new tools to study membrane-membrane or membrane-receptor interactions.

Citation Download Citation

John J. Pan and Deborah H. Charych "Molecular recognition and optical detection of biological pathogens at biomimetic membrane interfaces", Proc. SPIE 3040, Smart Structures and Materials 1997: Smart Materials Technologies, (14 February 1997); https://doi.org/10.1117/12.267115

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