Diffuse optical tomography of rat brain function

Andrew M. Siegel; John A. Marota; Joseph B. Mandeville; Bruce Rosen; David A. Boas

doi:10.1117/12.356815

15 July 1999 Diffuse optical tomography of rat brain function

Andrew M. Siegel, John A. Marota, Joseph B. Mandeville, Bruce Rosen, David A. Boas

Proceedings Volume 3597, Optical Tomography and Spectroscopy of Tissue III; (1999) https://doi.org/10.1117/12.356815
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

Diffuse optical tomography (DOT) can image spatial variations in highly scattering, tissue-like optical media. We have built an inexpensive and portable continuous-wave DOT system containing 32 laser sources (16 at 780nm and 16 at 830nm) and 16 detectors, which can acquire 288 independent measurements in less than 1 second. These data can then be processed using a variety of imaging algorithms. Preliminary studies have shown that this system can image brain bleeds in piglets, modulation of cerebral hemodynamics in rats, and brain function in both neonate and adult humans. The technical challenges involved in performing DOT over large optode areas is discussed. We describe the instrument and discuss a number of the technical issues which influenced its design. We then present a study of rat brain functional response to electrical forepaw stimulation measured with DOT, and compare it to functional MRI (fMRI). fMRI can separately measure blood volume, blood flow, and deoxy-hemoglobin concentration, and is thus a good benchmark for DOT. The relative performance of DOT and fMRI will be discussed. Our comparison shows similar temporal and spatial trends in blood volume and oxygen saturation following functional activation. These results clearly demonstrate the capabilities of DOT and set the stage for advancement to quantitative functional brain imaging.

Citation Download Citation

Andrew M. Siegel, John A. Marota, Joseph B. Mandeville, Bruce Rosen, and David A. Boas "Diffuse optical tomography of rat brain function", Proc. SPIE 3597, Optical Tomography and Spectroscopy of Tissue III, (15 July 1999); https://doi.org/10.1117/12.356815

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