Implementation of novel optical detection methods for clinically important blood analytes using microfabricated flow structures (T-Sensors)

Eric H. Altendorf; Bernhard H. Weigl

doi:10.1117/12.346743

21 April 1999 Implementation of novel optical detection methods for clinically important blood analytes using microfabricated flow structures (T-Sensors)

Eric H. Altendorf, Bernhard H. Weigl

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Proceedings Volume 3603, Systems and Technologies for Clinical Diagnostics and Drug Discovery II; (1999) https://doi.org/10.1117/12.346743
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

T-Sensors enable the optical detection of clinically significant analytes directly in whole blood. In microfluidic channels, fluids usually show laminar behavior. This allows the movement of different fluidic layers in a channel without mixing other than by diffusion. A sample solution, a detection solution, and a reference solution are introduce din a common channel. Smaller particles such as ions or small proteins diffuse rapidly across the fluid boundaries, whereas larger molecules diffuse more slowly. Large particles show no significant diffusion within the time the flow streams are in contact. Two interface zones are formed between the fluidic layers. The ratio of a property of the two interface zones is a function of the concentration of the analyte. In this paper, we introduce a novel optical geometry to determine absorbance or fluorescence at a number of distinct wavelength ranges, and to perform spectroscopic measurements with 1D or 2D spatial. Resolution in a flow channel. A flow channel is coupled to an optical filter with variable transmission in one or two dimensions, a light source, and a CCD detector. Such a device allows, for example, the absorption- or fluorescence- based detection of a variety of analytes in a T-sensor using a non-color-sensitive spatial detector, while retaining diffusion and reference information provided by the T-sensor principle.

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Eric H. Altendorf and Bernhard H. Weigl "Implementation of novel optical detection methods for clinically important blood analytes using microfabricated flow structures (T-Sensors)", Proc. SPIE 3603, Systems and Technologies for Clinical Diagnostics and Drug Discovery II, (21 April 1999); https://doi.org/10.1117/12.346743

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KEYWORDS

Optical filters

Diffusion

Image filtering

Linear filtering

Statistical analysis

Sensors

Light sources

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