Extension of SALS to transmural quantitative structural analysis of planar tissues

Michael S. Sacks; Xiaotong Lin

doi:10.1117/12.349193

1 June 1999 Extension of SALS to transmural quantitative structural analysis of planar tissues

Michael S. Sacks, Xiaotong Lin

Proceedings Volume 3604, Optical Diagnostics of Living Cells II; (1999) https://doi.org/10.1117/12.349193
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

Planar fibrous connective tissues are composed of a dense extra-cellular network of collagen and elastin fibers embedded in a ground matrix. Thus, quantification of fiber architecture an important step in developing an understanding of the mechanics of planar tissues. We have extensively used small angle light scattering (SALS) to map the gross fiber orientation of several soft membrane connective tissues using a custom built high speed mapping instrument. However, the current technique is limited to total through-thickness tissue structural analysis. The current study was undertaken to determine the feasibility of obtaining transmural tissue structural information from 2D SALS data. Methods: The basic approach is to utilize precisely aligned serial histological sections cut en-face through a tissue block and obtain 2D fiber structure from each section using SALS. Transumural fiber structure information is then derived by integration of 2D data to form a single data set containing the complete transmural fiber structure. To demonstrate the feasibility of the method, both explanted bioprosthetic heart valve (BHV) and native bovine pericardium (BP) tissues were evaluated. Results: The transmural SALS technique revealed for explanted BHV preferential damage in the fibrosa layer, while for BP variations in transmural fiber architecture were found consistent with optical histology. Conclusions: The transmural SALS technique successfully demonstrated quantitative transmural variations in fiber architecture in two dense collagenous tissues in a rapid, cost-effective approach.

Citation Download Citation

Michael S. Sacks and Xiaotong Lin "Extension of SALS to transmural quantitative structural analysis of planar tissues", Proc. SPIE 3604, Optical Diagnostics of Living Cells II, (1 June 1999); https://doi.org/10.1117/12.349193

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available