Scatter imaging of injured brain slices:  detection of mitochondrial injury

Lee J. Johnson; Daniel F. Hanley; Nitish V. Thakor

doi:10.1117/12.349207

1 June 1999 Scatter imaging of injured brain slices: detection of mitochondrial injury

Lee J. Johnson, Daniel F. Hanley, Nitish V. Thakor

Proceedings Volume 3604, Optical Diagnostics of Living Cells II; (1999) https://doi.org/10.1117/12.349207
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

Stroke has been shown to cause exitotoxic injury, two of its manifestations being cellular and mitochondrial swelling. In vitro models of stroke attempt to reproduce the effects of stroke by treating brain tissue with excitotoxins or hypotonic solutions. To further resolve the mechanism of stroke injury, we have designed a dual-angle scatter imaging (DASI) system sensitive to particle size. The DASI system has been used with a hippocampal slice preparation to contrast cellular swelling, induced by hypotonicity, and combined cellular and mitochondrial swelling caused by excitotoxicity. We found that both hypotonic end excitotoxic treatments caused changes in light scatter. However, only excitotoxic treatment caused a significant change in DASI.

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Lee J. Johnson, Daniel F. Hanley, and Nitish V. Thakor "Scatter imaging of injured brain slices: detection of mitochondrial injury", Proc. SPIE 3604, Optical Diagnostics of Living Cells II, (1 June 1999); https://doi.org/10.1117/12.349207

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KEYWORDS

Light scattering

Particles

Injuries

Brain

Imaging systems

Mie scattering

Scattering

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