Color segmentation of biological microscopic images

Pascal Lescure; Vannary Meas-Yedid; Henri Dupoisot; Georges Stamon

doi:10.1117/12.341119

9 March 1999 Color segmentation of biological microscopic images

Pascal Lescure, Vannary Meas-Yedid, Henri Dupoisot, Georges Stamon

Proceedings Volume 3647, Applications of Artificial Neural Networks in Image Processing IV; (1999) https://doi.org/10.1117/12.341119
Event: Electronic Imaging '99, 1999, San Jose, CA, United States

Abstract

The project consists in extracting biological objects from the background of an image in order to determine their three dimensions, namely their thickness. The small size of the photographed objects induces the formation of light interferences. The observed interference colors are related to the properties of the thin objects. Segmentation techniques used for this application are divided into three major types: edge extraction, region growing and splitting, clustering. Generally, edge segmentation works on each separated RGB channel but it leads to a data fusion problem Region growing and splitting methods commonly deal with features extraction. Color is a possible feature. The color image segmentation can be either monodimensional or multidimensional, using classification methods. For the monodimensional segmentation, the gray level is used alone. For the multidimensional case, one can take into account the vectorial character of colors, using color clustering. In this general context the aim of the project is to evaluate how a specific color space can improve the segmentation. Standard color segmentation algorithms are used: (1) C- means; (2) Back-propagation neural network; (3) Learning Vector Quantization. The results are compared with gray level algorithms such as the Otsu thresholding and ISODATA. Applied to each color channel. They show first that there is not only one good color representation space, and secondly, that data clusters are relatively close to each other, which explains why segmentation is so difficult in this class of pictures.

Citation Download Citation

Pascal Lescure, Vannary Meas-Yedid, Henri Dupoisot, and Georges Stamon "Color segmentation of biological microscopic images", Proc. SPIE 3647, Applications of Artificial Neural Networks in Image Processing IV, (9 March 1999); https://doi.org/10.1117/12.341119

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