Mechanism of low-level laser therapy (LLLT) effects on rat mast cells

Gennady K. Popov; Ludmila I. Solovyova; Arnold I. Kosel

doi:10.1117/12.405909

3 November 2000 Mechanism of low-level laser therapy (LLLT) effects on rat mast cells

Gennady K. Popov, Ludmila I. Solovyova, Arnold I. Kosel

Proceedings Volume 4159, Effects of Low-Power Light on Biological Systems V; (2000) https://doi.org/10.1117/12.405909
Event: EOS/SPIE European Biomedical Optics Week, 2000, Amsterdam, Netherlands

Abstract

The low power laser radiation is widely applied for treatment of various diseases. In our research we investigated the influence of low power laser radiation on the mast cells degranulation process. The object of the research were the mesentery mast cells of the rat thin intestine. A loop of thin intestine was irradiated by the therapeutic diode laser device Uley - 2K (lambda - 890 nm, pulse). The process of mast cells degranulation served as a criterion for their functional activity estimation. The estimation was fulfilled with the help of light microscope (toluidine blue staining, pH02,0; degranulating mast cells counting on 100 cells; immersion technique; X 980). To study the dependence of degranulation process of mast cells irradiated with lasre from intracellular calcium (Ca²⁺) concentration we applied 0,000015 M solution of verapamil, which was applied to the mesentery for 2 minutes. Laser radiation (890 nm) stimulates mesentery mast cells degranulation. This effect is dose-dependent. Maximal degranulation was registered after laser irradiation wiht power 25 mW, exposure time 15-30 s (energy density 7.5 x 10³ J/m² to 6 x 10⁴ j/m²). Further increasing of exposure time caused the effect decreasing. The results of our experiments with verpamil let us suppose light interaction with the voltage-dependent subunit of calcium channel, changing intracellular Ca²⁺ and leading to stimulatory effects.

Citation Download Citation

Gennady K. Popov, Ludmila I. Solovyova, and Arnold I. Kosel "Mechanism of low-level laser therapy (LLLT) effects on rat mast cells", Proc. SPIE 4159, Effects of Low-Power Light on Biological Systems V, (3 November 2000); https://doi.org/10.1117/12.405909

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