Role of PKC isozymes in low-power light-stimulated proliferation of cultured skin cells

Nili Grossman; Vered Kleitman; Julia Meller; Roland Kaufmann; Nermin Akgun; Angelika Ruck; Etta Livneh; Rachel Lubart

doi:10.1117/12.405908

3 November 2000 Role of PKC isozymes in low-power light-stimulated proliferation of cultured skin cells

Nili Grossman, Vered Kleitman, Julia Meller, Roland Kaufmann, Nermin Akgun, Angelika Ruck, Etta Livneh, Rachel Lubart

Proceedings Volume 4159, Effects of Low-Power Light on Biological Systems V; (2000) https://doi.org/10.1117/12.405908
Event: EOS/SPIE European Biomedical Optics Week, 2000, Amsterdam, Netherlands

Abstract

Exposure of cultured skin cells to low power visible light leads to a transiently stimulated proliferation. Facilitation of this response requires the presence of active PKC, elevation of intracellular calcium, and involves reactive oxygen species. In the present study, the role of PKC(alpha) and PCK(eta) was examined using paired murine fibroblasts, differing in the level of these isozymes expression. The ability of the cells to respond to low power UVA light or HeNe laser by stimulated proliferation was correlated with an active state or overexpression of PKC(alpha) , but not PKC(eta) . A parallel response was obtained in cells that were loaded with A1PcS₄ before photosensitization. Whenever this latter treatment caused a light-stimulated inhibition, it was accompanied by the intracellular calcium and photosensitizer dynamics typical of the effect of PDT on rate epithelial cells. Accordingly, added antioxidants that suppressed light-stimulated proliferation also suppressed this light-stimulated inhibition. The model systems employed in this study are the first to demonstrate the specific effect of PKC isozymes on light-stimulated proliferation, in relation to oxidative stress, and indicate their dual role in light-tissue interaction.

Citation Download Citation

Nili Grossman, Vered Kleitman, Julia Meller, Roland Kaufmann, Nermin Akgun, Angelika Ruck, Etta Livneh, and Rachel Lubart "Role of PKC isozymes in low-power light-stimulated proliferation of cultured skin cells", Proc. SPIE 4159, Effects of Low-Power Light on Biological Systems V, (3 November 2000); https://doi.org/10.1117/12.405908

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