Novel approach for the rapid discrimination of bacterial strains by Fourier transform near-infrared spectroscopy

Luis E. Rodriguez-Saona; Farukh M. Khambaty; Francis S. Fry; Elizabeth M. Calvey

doi:10.1117/12.418739

13 March 2001 Novel approach for the rapid discrimination of bacterial strains by Fourier transform near-infrared spectroscopy

Luis E. Rodriguez-Saona, Farukh M. Khambaty, Francis S. Fry, Elizabeth M. Calvey

Author Affiliations +

Proceedings Volume 4206, Photonic Detection and Intervention Technologies for Safe Food; (2001) https://doi.org/10.1117/12.418739
Event: Environmental and Industrial Sensing, 2000, Boston, MA, United States

Abstract

To address the need for a fast and sensitive method for the detection of bacterial contamination in solutions, we evaluated the use of Fourier-transform near infrared (FT-NIR) spectroscopy and multivariate pattern recognition techniques. The complex cellular composition of bacteria yields FT-NIR vibrational transitions (overtone and combination bands) that might be used for identification and sub-typing. Bacterial suspensions (E. coli HB1O1, E. coli ATCC43888, E. coli 1224, Pseudomonas aeruginosa, Bacillus cereus and Listeria innocua) were filtered to concentrate the cells and eliminate the matrix which has a strong NIR signal. FT-NIR measurements were done by using a diffuse reflection-integrating sphere. The use of a simple membrane filtration procedure to produce a thin, uniform bacterial film generated reproducible FT-NIR spectra that can be used for rapid discrimination among closely related strains. Transformation ofthe spectra with second derivatives resolved specific FT-NIR features in the information-rich spectral region of 5000-4000 cm-1 to allow principal components analysis to group the samples into different tight clusters. The use ofAnodisc membranes gave the more reproducible results. This methodology appears promising for the rapid evaluation of potential bacterial contamination in liquids with minimal sample manipulation.

Citation Download Citation

Luis E. Rodriguez-Saona, Farukh M. Khambaty, Francis S. Fry, and Elizabeth M. Calvey "Novel approach for the rapid discrimination of bacterial strains by Fourier transform near-infrared spectroscopy", Proc. SPIE 4206, Photonic Detection and Intervention Technologies for Safe Food, (13 March 2001); https://doi.org/10.1117/12.418739

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available