Effect of HeNe laser irradiation on the phagocytosis of macrophages in the immune organs of mice

Mingji Ren; Yonghong Shi; Jianwei Wang; Weizhong Yuan

doi:10.1117/12.403949

11 October 2000 Effect of HeNe laser irradiation on the phagocytosis of macrophages in the immune organs of mice

Mingji Ren, Yonghong Shi, Jianwei Wang, Weizhong Yuan

Proceedings Volume 4224, Biomedical Photonics and Optoelectronic Imaging; (2000) https://doi.org/10.1117/12.403949
Event: Optics and Optoelectronic Inspection and Control: Techniques, Applications, and Instruments, 2000, Beijing, China

Abstract

In order to study the effect of HeNe laser on macrophages phagocytosis, the are over liver and spleen of the mice was irradiated with NeHe laser at the dosage of 63.7J/cm² for 3,5,7, and 10 days respectively, 5 min each day, then observing the phagocytosis of macrophages of the immune organs after trypan blue injecting live mice, and made quantitative analysis of macrophages phagocytosis by image analysis system. The results showed that the four indexes: number of trypan blue granule (GN), area of granule (GA), ratio of granule area to cell area (GA/CA) and granule integral optical density (IOD) at irradiated groups were higher than that of control group (P<0.01). Comparison between different irradiated groups were higher than that of control group (P<0.01). The study indicated that HeNe laser with appropriate dosage can activate macrophages of the immune organs, and enhance their organic immunity.

Citation Download Citation

Mingji Ren, Yonghong Shi, Jianwei Wang, and Weizhong Yuan "Effect of HeNe laser irradiation on the phagocytosis of macrophages in the immune organs of mice", Proc. SPIE 4224, Biomedical Photonics and Optoelectronic Imaging, (11 October 2000); https://doi.org/10.1117/12.403949

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