Computer-controlled laser ablation: a novel tool for biomolecular patterning

Jonathan P. Wright; Chitladda Mahanivong; Duy K. Pham; Dan V. Nicolau; K. Suyama; Masamitsu Shirai; Masahiro Tsunooka

doi:10.1117/12.454620

19 November 2001 Computer-controlled laser ablation: a novel tool for biomolecular patterning

Jonathan P. Wright, Chitladda Mahanivong, Duy K. Pham, Dan V. Nicolau, K. Suyama, Masamitsu Shirai, Masahiro Tsunooka

Proceedings Volume 4590, BioMEMS and Smart Nanostructures; (2001) https://doi.org/10.1117/12.454620
Event: International Symposium on Microelectronics and MEMS, 2001, Adelaide, Australia

Abstract

This paper describes a novel laser-based method for preparing microchannels in a bilayer system consisting of a UV sensitive polymer, acetophenone O-acryloyloxime (AAPO), layered with bovine serum albumin (BSA); BSA acts as a common blocking agent to prevent biomolecular attachment to the unexposed regions. The focus of the paper is on the use of a computer-controlled laser ablation system comprising a research-grade inverted optical microscope, a pulsed nitrogen laser emitting at 337 nm and a programmable X-Y-Z stage. By using a 100x oil immersion objective, channels of 1micrometers width and ca. 1 mm depth can be etched into the BSA-coated polymer. The precise width of the channel can be controlled by simply adjusting both the laser power and focusing. The addition of myosin to the base of these channels provides tracks on which actin filaments can move. By adjusting the width of the tracks, it is possible to regulate the direction of motion of the actin filaments.

Citation Download Citation

Jonathan P. Wright, Chitladda Mahanivong, Duy K. Pham, Dan V. Nicolau, K. Suyama, Masamitsu Shirai, and Masahiro Tsunooka "Computer-controlled laser ablation: a novel tool for biomolecular patterning", Proc. SPIE 4590, BioMEMS and Smart Nanostructures, (19 November 2001); https://doi.org/10.1117/12.454620

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available